Journal: Respiratory Research

Article Title: Comprehensive profiling of lactylation-associated genes in pulmonary hypertension through bulk and single-cell RNA sequencing integration

doi: 10.1186/s12931-025-03446-9

Figure Lengend Snippet: Single-cell RNA sequence analysis. A Lactate content in hPASMCs under hypoxic conditions. B Lactate content in hPASMCs under PDGF-BB stimulation conditions. C Hypoxia induces global and site-specific histone lactylation in hPASMCs. D Verification of mRNA expression of LCP1 in HPH. E Verification of mRNA expression of LCP1 in PAH F . Verification of protein expression of LCP1 in HPH. G Verification of protein expression of LCP1 in PAH. H Cell proliferation assessed by EdU assay. I Apoptpsis assessed by flow cytometry. * p < 0.05 vs normoxia; ** p < 0.01 vs normoxia; # p < 0.05 vs control; ## p < 0.01 vs control

Article Snippet: For drug-induced PAH modeling, Platelet-Derived Growth Factor-BB (PDGF-BB) (MCE, New Jersey, USA) was administered to hPASMCs at a concentration of 20 ng/mL.

Techniques: Sequencing, Expressing, EdU Assay, Flow Cytometry, Control